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R8759

Sigma-Aldrich

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

Type X-SA, lyophilized powder

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
MDL number:
NACRES:
NA.51

type

Type X-SA

Quality Level

form

lyophilized powder

storage temp.

−20°C

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Application

Ribonucleic acid, transfer from baker′s yeast S. cerevisiae may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Unit Definition

One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).

Preparation Note

Prepared by a modification of the procedure of Hancher, C.W., et al., Biotechnol. Bioeng., 11, 1055 (1969).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Ofri Levi et al.
Nucleic acids research, 49(1), 432-443 (2020-12-12)
Modification of nucleotides within an mRNA emerges as a key path for gene expression regulation. Pseudouridine is one of the most common RNA modifications; however, only a few mRNA modifiers have been identified to date, and no one mRNA pseudouridine
Maureen A Sartor et al.
Nucleic acids research, 34(1), 185-200 (2006-01-07)
The recent sequencing of a large number of Xenopus tropicalis expressed sequences has allowed development of a high-throughput approach to study Xenopus global RNA gene expression. We examined the global gene expression similarities and differences between the historically significant Xenopus
Jaclyn M Fingerhut et al.
PLoS genetics, 15(5), e1008028-e1008028 (2019-05-10)
Intron gigantism, where genes contain megabase-sized introns, is observed across species, yet little is known about its purpose or regulation. Here we identify a unique gene expression program utilized for the proper expression of genes with intron gigantism. We find
Violaine I Mottier-Pavie et al.
Developmental biology, 417(1), 50-62 (2016-07-02)
The mechanisms that modulate and limit the signaling output of adult stem cell niches remain poorly understood. To gain further insights into how these microenvironments are regulated in vivo, we performed a candidate gene screen designed to identify factors that
Peter Kozulin et al.
Molecular vision, 15, 2649-2662 (2009-12-17)
Recently we identified high levels of expression of Eph-A6 in the macula of developing human retina and showed localization of Eph-A6 to ganglion cells (GC). In the present study we investigated the expression of some members of the ephrin family

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