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R9782

RS-1

Name: RS-1
Brand: SIGMA

≥98% (HPLC), RAD51 stimulator, powder

Synonym(s):

3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide, 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₁₆Br₂N₂O₃S

CAS Number:

312756-74-4

Molecular Weight:

524.23

UNSPSC Code:

12352200

PubChem Substance ID:

329824048

NACRES:

NA.77

MDL number:

MFCD00348720

Assay:

≥98% (HPLC)

Form:

powder

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Properties

Product Name

RS-1, ≥98% (HPLC)

assay

≥98% (HPLC)

form

powder

color

off-white to light tan

solubility

DMSO: ≥10 mg/mL

storage temp.

room temp

SMILES string

Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1

InChI

1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

InChI key

SWKAVEUTKGKHSR-UHFFFAOYSA-N

Description

Application

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.

RS-1 has been used:

as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)
as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells
as RAD51 agonist to study its effects on double-stranded break repair

Biochem/physiol Actions

RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.

RS-1 is a stimulator of the human homologous recombination protein RAD51.

RS-1 is a sulfonamido-benzamide compound.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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