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RAB0083

Sigma-Aldrich

Human CD200 ELISA Kit

for serum, plasma, cell culture supernatant and urine

NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 24.58-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CD200(4345)

General description

The Human CD200 (also known as OX-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD200 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CD200

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

CD antigen 200 (CD200) acts as an immunosuppressor and is involved in maintaining immune homeostasis. The gene is overexpressed in a number of human cancers. The protein supports tumor growth and invasion by suppressing anti-tumor immune responses. CD200 is considered as a biomarker in colon cancer and cutaneous squamous cell carcinoma. Deficiency of CD200 results in hyper-activation of macrophages and enhanced immune response to autoimmune disease.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Identification of CD200+ colorectal cancer stem cells and their gene expression profile.
Zhang SS
Oncology Reports (2016)
Over-Expression of CD200 Predicts Poor Prognosis in Cutaneous Squamous Cell Carcinoma.
Li L
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research (2016)
A Truncated form of CD200 (CD200S) Expressed on Glioma Cells Prolonged Survival in a Rat Glioma Model by Induction of a Dendritic Cell-Like Phenotype in Tumor-Associated Macrophages.
Kobayashi K
Neoplasia (2016)
Monika Abramiuk et al.
Journal of clinical medicine, 9(9) (2020-09-25)
The causes of endometriosis (EMS) remain unknown; however, a number of immunological abnormalities contribute to the pathogenesis of the disease. The cluster of differentiation-200 (CD200) and its receptor (CD200R) maintain peripheral self-tolerance by negatively regulating immune responses. In this comparative
Characterization of CD200 Ectodomain Shedding.
Wong KK
PLoS ONE (2016)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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