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RAB0100

Sigma-Aldrich

Human Granulocye-Macrophage Colony Stimulating Factor / CSF2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):
GM-CSF
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 2.05-500 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CSF2(1437)

General description

The Human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GM-CSF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human GM-CSF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration. GM-CSF plays a crucial role in inflammatory and autoimmune diseases. In neutrophils, it promotes the expression of TLR2 (toll-like receptor) and TLR2-associated interleukin 8 responses, thereby eliminating microbial attack.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Rapamycin inhibits GM-CSF-induced neutrophil migration.
Gomez-Cambronero J
Febs Letters (2003)
GM-CSF in inflammation and autoimmunity.
Hamilton JA
Trends in Immunology (2002)
Association of genetic variations in the CSF2 and CSF3 genes with lung function in smoking-induced COPD.
The European Respiratory Journal (2008)
Agnieszka Swierczak et al.
Cancer immunology research, 2(8), 765-776 (2014-07-10)
Treatment options are limited for patients with breast cancer presenting with metastatic disease. Targeting of tumor-associated macrophages through the inhibition of colony-stimulating factor-1 receptor (CSF-1R), a key macrophage signaling pathway, has been reported to reduce tumor growth and metastasis, and
Growth of clonogenic myeloblastic leukemic cells in the presence of human recombinant erythropoietin in addition to various human recombinant hematopoietic growth factors.
Asano Y
Blood (1988)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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