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RAB0143

Sigma-Aldrich

Human Dkk-1 ELISA Kit

for serum, plasma, cell culture supernatant and urine

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 122.9-30000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DKK1(22943)

General description

The human Dkk-1 (hDkk-1) gene, a transcriptional target of the p53 tumor suppressor, encodes a powerful inhibitor of the Wnt signaling pathway and regulates the spatial patterning/morphogenesis of the mammalian central nervous system.The Dkk-1 gene, also called Dickkopf-1 , is a prototype member of a recently identified gene family that encodes secreted glycoproteins that control cell fate and neural patterning during embryonic development. This gene is located on human chromosome 10q.

Immunogen

Recombinant Human DKK1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Evidence strongly indicates that Dkk-1 protein functions as a potent inhibitor of the Wnt signaling pathway to enable appropriate positioning and development of the embryonic brain and other organ structures. hDkk-1 protein also has a pro-apoptotic function.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Human Dkk-1, a gene encoding a Wnt antagonist, responds to DNA damage and its overexpression sensitizes brain tumor cells to apoptosis following alkylation damage of DNA
Shou J, et al.
Oncogene, 21(6), 878-878 (2002)
Tumors of the Central Nervous System (2011)
Mariusz L Hartman et al.
PloS one, 9(4), e95157-e95157 (2014-04-16)
The diversity of functional phenotypes observed within a tumor does not exclusively result from intratumoral genetic heterogeneity but also from the response of cancer cells to the microenvironment. We have previously demonstrated that the morphological and functional phenotypes of melanoma

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