RAB0197
Human Ferritin ELISA Kit
for serum, plasma, cell culture supernatant and urine
Synonym(s):
FTH, Ferritin H subunit, Ferritin heavy chain
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... FTH1(2495)
Input:
sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Product Name
Human Ferritin ELISA Kit, for serum, plasma, cell culture supernatant and urine
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FTH1(2495)
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).
Biochem/physiol Actions
Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.
Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.
General description
The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).
Immunogen
Recombinant Human Ferritin
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
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shRNA targeting of ferritin heavy chain activates H19/miR-675 axis in K562 cells.
Di Sanzo M, et al.
Gene, 657, 92-99 (2018)
Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.
Kimura T, et al.
The Embo Journal, e201695081-e201695081 (2016)
Nishant Kumar Ojha et al.
Molecular and cellular biochemistry, 417(1-2), 75-85 (2016-05-14)
Hepatitis E Virus (HEV) is the major causative agent of acute hepatitis in developing countries. Its genome has three open reading frames (ORFs)-called as ORF1, ORF2, and ORF3. ORF1 encodes nonstructural polyprotein having multiple domains, namely: Methyltransferase, Y domain, Protease
In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch
He X, et al.
Oncotarget, 7(48), 78591-78591 (2016)
Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis.
Aversa I, et al.
Journal of Experimental & Clinical Cancer Research, 36(1), 104-104 (2017)
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