CN
All Photos(2)

RAB0252

Sigma-Aldrich

Human IL-12 p70 ELISA Kit

for serum, plasma, cell culture supernatant and urine

NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.8-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL12A(3592)

General description

The Human IL-12(P70) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P70) in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IL-12 p70

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IL-12 p70 ELISA kit has been used to determine the concentrations of IL-12, IL-10, IL-1β and TNFα in cell culture supernatants.

Biochem/physiol Actions

IL-12A (interleukin 12 A) is essential for differentiation of Th1 (type 1) cells. Polymorphisms in IL-12A gene is associated with Graves′ and Alzheimer′s disease, rheumatoid arthritis and asthma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Interleukin 35 polymorphisms are associated with decreased risk of premature coronary artery disease, metabolic parameters, and IL-35 levels: the genetics of atherosclerotic disease (GEA) study.
Posadas-Sanchez R, et al.
Mediators of Inflammation, 2017, 10-10 (2017)
Brucella Omp25 Upregulates miR-155, miR-21-5p, and miR-23b to Inhibit Interleukin-12 Production via Modulation of Programmed Death-1 Signaling in Human Monocyte/Macrophages.
Cui B, et al.
Frontiers in Immunology, 8, 708-708 (2017)
Jianfei Ji et al.
Stem cells translational medicine, 3(3), 356-364 (2014-01-01)
Targeting cancer stem cells (CSCs) with immunotherapy may be an effective means to prevent recurrences in glioblastoma multiforme (GBM). It is well established that CD133 is expressed in the population of GBM tumor cells representing CSCs. This raises a possibility
Andrea L Armstead et al.
International journal of nanomedicine, 11, 6195-6206 (2016-12-07)
Identifying the toxicity of nanoparticles (NPs) is an important area of research as the number of nanomaterial-based consumer and industrial products continually rises. In addition, the potential inflammatory effects resulting from pulmonary NP exposure are emerging as an important aspect
Jin-Sung Chung et al.
The Journal of investigative dermatology, 134(11), 2784-2794 (2014-06-18)
A major barrier to successful cancer immunotherapy is the tumor's ability to induce T-cell tolerance by exploiting host regulatory mechanisms. Having discovered the DC-HIL receptor, which inhibits T-cell responses by binding to syndecan-4 on effector T cells, we posited the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service