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RAB0282

Sigma-Aldrich

Equine Interleukin-1 Receptor Antagonist / IL-1F3 ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):
IL-1ra
NACRES:
NA.32

Quality Level

species reactivity

equine

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 12.29-3000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

General description

The Equine IL-1ra (interleukin 1 receptor antagonist) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of equine IL-1ra in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Equine IL-1 Receptor Antagonist

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α and IL-1β by competitively binding to their type I and type II receptors. IL-1RA plays a vital role in inhibition of acute pro-inflammatory response and forbidding chronic inflammation from damaging healthy tissues. Abnormal expression of IL-1RA leads to development of inflammatory or autoimmune related diseases. Allelic polymorphism of IL-1Rα leads to inflammatory bowel disease (IBD). Therapeutically, IL-1Rα may help in the treatment of sepsis, rheumatoid arthritis, asthma. Overexpression of IL-1Rα is observed in serum of psoriatic arthritis (PsA) patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Relationship of plasma leptin to plasma cytokines and human survivalin sepsis and septic shock.
Arnalich F, et al.
The Journal of Infectious Diseases, 180(3), 908-911 (1999)
Expression of interleukin-1 beta (IL-1beta) and interleukin-1 receptor antagonist (IL-1ra) on asthmatic bronchial epithelium.
Sousa A R, et al.
American Journal of Respiratory and Critical Care Medicine, 154(4 Pt 1), 1061-1066 (1996)
Serum levels of IL-10, IL-6, IL-1ra, and sIL-2R in patients with psoriatic arthritis.
Elkayam O, et al.
Rheumatology International, 19(3), 101-105 (2000)
Treatment of rheumatoid arthritis with recombinant human interleukin-1 receptor antagonist.
Bresnihan B, et al.
Arthritis and Rheumatism, 41(12), 2196-2204 (1998)
A novel association between IL1-Ra (receptor antagonist) gene polymorphism and T1DM in Al-Madina Al-Mounawra.
Ali RA
European Review for Medical and Pharmacological Sciences, 19(19), 3701-3708 (2015)

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