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RAB0320

Sigma-Aldrich

Human IL-8 / CXCL8 ELISA Kit

for cell and tissue lysates

NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.8-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL8(3576)

General description

The Human IL-8 ELISA (enzyme-linkedimmunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human interleukin-8 (IL-8) cell lysate and tissue lysate. Elevated expression of the gene is observed in a number of cancers including chronic spontaneous urticaria and breast cancer, thus serves as a prognostic marker for the same. IL-8 regulates the metastasis and angiogenesis processes during cancer development.Itis responsible for epithelial-mesenchymal transition which plays an important role in providing cancer cells the mobility to invade other neighbouring cells, thereby contributing to metastasis.

Immunogen

Recombinant Human IL-8

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IL-8/CXCL8 ELISA Kit has been used to determine serum concentrations of interleukin-8.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABLYSIS12X Cell Lysis Buffer (Item J)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Dioscoreophyllum cumminsii (Stapf) Diels leaves halt high-fructose induced metabolic syndrome: Hyperglycemia, insulin resistance, inflammation and oxidative stress
Ajiboye TO, et al.
Journal of Ethnopharmacology, 192, 471-479 (2016)
Aqueous seed extract of Hunteria umbellata (K. Schum.) Hallier f.(Apocynaceae) palliates hyperglycemia, insulin resistance, dyslipidemia, inflammation and oxidative stress in high-fructose diet-induced metabolic syndrome in rats
Ajiboye TO, et al.
Journal of Ethnopharmacology, 198, 184-193 (2017)
Elevated plasma il-8 concentration is related to severity of systemic inflammation in chronic spontaneous urticaria.
Kasperska ZA, et al.
Journal of Biological Regulators and Homeostatic Agents, 31(4), 957-961 null
Sohlh2 suppresses epithelial to mesenchymal transition in breast cancer via downregulation of IL-8.
Ji S, et al.
Oncotarget, 7(31), 49411-49411 (2016)
Tara M Weitz et al.
The American journal of pathology, 184(11), 2855-2861 (2014-09-02)
Activated microglia are associated with amyloid plaques in transgenic mouse models of cerebral amyloidosis and in human Alzheimer disease; yet, their implication in Alzheimer disease pathogenesis remains unclear. It has been suggested that microglia play dual roles depending on the

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