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RAB0321

Sigma-Aldrich

Porcine IL-8 ELISA Kit

for serum, plasma and cell culture supernatant

NACRES:
NA.32

species reactivity

porcine

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.19-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

Porcine ... IL8(396880)

General description

The Porcine IL-8 (chemokine, interferon γ-inducible T cell α chemoattractant) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-8 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Porcine IL-8

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Porcine interleukin-8 (IL-8) is a cytokine, exhibiting inflammatory action in lungs. It is known to stimulate neutrophils, in response to lung damage. Increased levels of IL-8 is observed in fibroblasts, monocytes, and hepatocytes during inflammation.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 expression through TAK-1/JNK/AP-1 pathways.
Liu, Y, et al.
Virology, 506 (2017)

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