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RAB0327

Human Insulin ELISA Kit

Name: Human Insulin ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Insulin

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About This Item

NACRES:

NA.32

UNSPSC Code:

41116158

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69-300 μIU/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INS(3630)

Description

General description

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Insulin

Application

Human Insulin ELISA Kit has been used:

to measure the blood levels of human insulin and proinsulin in the transgenic mice
to measure serum insulin indaibetic rats
to measure insulin in Multiple Sclerosis patients

For research use only. Not for use in diagnostic procedures.

Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

RABELADA
ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
SDS

RABELADB
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
SDS

RABSTOP3
ELISA Stop Solution (Item I)
SDS

RABTMB3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
SDS

RABWASH4
20X Wash Buffer (Item B)
SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品

低风险生物材料

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