Skip to Content
Merck
CN

RAB0334

Mouse Leptin ELISA Kit

for serum, plasma, cell culture supernatant

Synonym(s):

Leptin, Obese protein, Obesity factor, OB

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Lep(16846)

General description

The Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Leptin in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse Leptin

Application

For research use only. Not for use in diagnostic procedures.

Mouse Leptin ELISA Kit has been used to measure plasma leptin concentration.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse Leptin ELISA kit has been used to measure serum leptin concentration in adipose tissues of mice.

Biochem/physiol Actions

Leptin is a hormone secreted by adipocytes. It is majorly involved in regulation of food intake and energy balance. Variation in the leptin promoter alters serum leptin and HDLC concentration, which might lead to development of obesity. Increased concentration of serum leptin causes arterial stiffness in coronary artery disease (CAD) patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

Regulatory Information

低风险生物材料
常规特殊物品

This item has



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Leptin promoter variant G2548A is associated with serum leptin and HDL-C levels in a case control observational study in association with obesity in a Pakistani cohort.
Shabana and Hasnain S
Journal of Biosciences null
Redistribution of tissue zinc pools during lactation and dyshomeostasis during marginal zinc deficiency in mice.
McCormick NH
Journal of Trace Elements in Medicine and Biology null
Janaka Wickramasinghe et al.
Animals : an open access journal from MDPI, 12(12) (2022-06-25)
The study objective was to examine the effects of supplementing Gln and BCAA on the SFI and ADG of weaning dairy calves. Holstein heifer calves (11 calves /treatment) at 35 d of age were assigned to: (1) no amino acids