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RAB0465

Sigma-Aldrich

Human Tie-1 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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NACRES:
NA.32

Quality Level

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.68-500 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIE1(7075)

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RAB0457RAB0501RAB0273
packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma
sample type urine

input

sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.68-500 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 20.58-15000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 70 pg/mL
standard curve range: 68.59-50000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 pg/mL
standard curve range: 0.48-100 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human Tie-1 (Tyrosine Kinase with Ig and EGF homology domain 1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Tie-1 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TIE1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Regulatory Information

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