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Merck
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RAB1151

Rat OPN ELISA Kit

for cell culture supernatants, plasma, and serum samples

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About This Item

NACRES:
NA.84
UNSPSC Code:
41116158
Gene information:
rat ... Spp1(25353)
Input:
sample type serum sample(s)
sample type plasma
sample type cell culture supernatant(s)
Species reactivity:
mouse, rat
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum sample(s)
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/ml

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Spp1(25353)

General description

This ELISA antibody pair detects rat and mouse OPN. Other species are not determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Shirley T Padilla et al.
Biomolecules, 10(11) (2020-11-05)
Acquired immunodeficiency syndrome (AIDS) complicated with tuberculosis (TB) is a global public issue. Due to the paucity of bacteria in AIDS/TB, blood-based biomarkers that reflect disease severity are desired. Plasma levels of matricellular proteins, such as osteopontin (OPN) and galectin-9
Jessica F Olive et al.
PloS one, 13(6), e0198790-e0198790 (2018-06-14)
Gene editing protocols often require the use of a subcloning step to isolate successfully edited cells, the behavior of which is then compared to the aggregate parental population and/or other non-edited subclones. Here we demonstrate that the inherent functional heterogeneity
Yuta Kawahara et al.
Brain & development, 42(2), 185-191 (2019-12-04)
The pathogenesis of acute encephalopathy (AE) remains unclear, and a biomarker has not been identified. Levels of 49 cytokines and chemokines, including osteopontin (OPN), were measured in serum and cerebrospinal fluid (CSF) of children with AE (n = 17) or febrile convulsion
Tobias Zehnder et al.
Biofabrication, 9(2), 025016-025016 (2017-03-08)
Biofabrication aims to develop functional, biological constructs using automated processes (additive manufacturing, AM) involving different cell types and biomaterials (Groll  et al 2016  Biofabrication 13001 1-6). As bone tissue is based on the crosstalk between osteoblasts and osteoclasts at least
Machiko Honda et al.
Journal of cell communication and signaling, 14(2), 223-232 (2020-02-18)
Previously, we reported that an extracellular matrix protein, osteopontin (OPN), is involved in various autoimmune diseases using a neutralizing polyclonal antibody against OPN generated in rabbits. However, the antibody cannot be used for long-term mouse models of chronic inflammatory disease

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