RECOMT
Thrombin CleanCleave™ Kit
Synonym(s):
Thrombin Cleavage Kit
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352200
form
suspension
Quality Level
mol wt
37 kDa
technique(s)
protein purification: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Application
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Features and Benefits
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Preparation Note
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Legal Information
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Kit Components Only
Product No.
Description
- Thrombin Cleavage Buffer, 10× 10 mL
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Protocols
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| RECOMT-1KT | 04061835573950 |
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