RT7POL
T7 RNA Polymerase, for in vitro transcription
≥50,000 units/mL, aqueous solution
别名:
bacteriophage T7 RNA polymerase, T7 RNAP, e coli T7 RNA polymerase, in vitro mRNA polymerase, recombinant T7
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关于此项目
UNSPSC Code:
12352204
NACRES:
NA.42
Specific activity:
≥50000 units/mL
Assay:
≥95% (SDS-PAGE)
Biological source:
bacteriophage T7
Recombinant:
expressed in E. coli
Concentration:
≥50000 units/mL
Shelf life:
2 yr at -20 °C
产品名称
T7 RNA Polymerase, ≥50,000 units/mL, aqueous solution
biological source
bacteriophage T7
Quality Segment
recombinant
expressed in E. coli
grade
Molecular Biology
assay
≥95% (SDS-PAGE)
form
aqueous solution (containing 50% glycerol), liquid
specific activity
≥50000 units/mL
shelf life
2 yr at -20 °C
mol wt
11.4 kDa
storage condition
OK to freeze
concentration
≥50000 units/mL
color
colorless
optimum pH
7.7 (37 °C)
pH
7.9 (25 °C)
suitability
suitable for (In-vitro RNA transcription)
application(s)
research use
foreign activity
DNAse, none detected
RNAse, none detected
Nickase, none detected
shipped in
dry ice
storage temp.
-10 to -25°C
General description
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5′ to 3′ direction. The T7 polymerase also requires a double stranded DNA template and Mg2+ ion as cofactor for the synthesis of RNA. It has a very low error rate.
Application
This product is a recombinant protein expressed in E. coli and induced by IPTG. The MW of T7 RNA Polymerase is 98.9 kDa and theoretical pI of ~6.8.
Features and Benefits
T7 polymerase is extremely promoter-specific, transcribes only DNA downstream of a T7 promoter, and can be used for in vitro mRNA synthesis. Quality tests include:
- Nickase activty: None detected in a 50 µl reaction containing 250 ng of supercoiled pUC19 DNA and at least 30 units of T7 RNA Polymerase incubated for 4 hours at 37°C.
- DNase Activity: None detected in a 50 µl reaction containing 250 ng linear pUC19 DNA and at least 30 units of T7 RNA Polymerase incubated for 16 hours at 37°C.
- RNase Activity: None detected in a 50 µl reaction containing 1.25-2.5 ng tRNA and at least 30 units of T7 RNA Polymerase is incubated for 4h at 37°C.
Physical form
The product is supplied as an aqueous solution containing 50mM Tris-HCl, 100mM NaCl, 20mM β-ME, 1mM EDTA, 0.02% (w/v) Tween 20 and 50% glycerol, pH 7.9 at 25 °C.
Other Notes
1 Unit of T7 RNA polymerase is defined as the amount of enzyme that synthesizes 1ug of mRNA per hour in a 30-minute reaction containing 25μg/ml linearized plasmid, 3mM of each NTP and 18mM MgCl2, at 37°C, pH 7.7
存储类别
10 - Combustible liquids
wgk
WGK 2