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Merck
CN

S0698

Anti-SmB antibody, Mouse Monoclonal

clone 12F5, purified from hybridoma cell culture

Synonym(s):

Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-SmB antibody, Mouse Monoclonal, clone 12F5, purified from hybridoma cell culture

Quality Level

200

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

12F5, monoclonal

mol wt

antigen ~25 kDa

species reactivity

human, mouse, canine

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

P14678

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SNRPB(6628)
mouse ... Snrpb(20638)

Application

  • Monoclonal Anti-SmB antibody produced in mouse has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.
SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

Immunogen

SmB recombinant protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000415476
0000415476
0000413321
0000413321
0000377871

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Concerted regulation and molecular evolution of the duplicated SNRPB'/B and SNRPN loci
Gray TA, et al.
Nucleic Acids Research, 27(23), 4577-4584 (1999)
Smed-SmB, a member of the LSm protein superfamily, is essential for chromatoid body organization and planarian stem cell proliferation
Fernandez-TE, et al.
Development, 137(7), 1055-1065 (2010)
U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein
Skrajna A, et al.
RNA, 23(6), 938-951 (2017)
Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma
Correa BR, et al.
Genome Biology, 17(1) (2016)
SmD3 regulates intronic noncoding RNA biogenesis
Scruggs BS, et al.
Molecular and Cellular Biology, 32(20), 4092-4103 (2012)
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