Anti-Sheep IgG (whole molecule) antibody produced in rabbit

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.

purified sheep IgG

Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:

• Enzyme-linked immunosorbent assay (ELISA)
• Immunoprecipitation
• labelling electrodes in immunosensor systems

Binds all sheep Igs

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.

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