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S1797

SOC Medium

Name: SOC Medium
Brand: SIGMA

For use in transformation

Synonym(s):

Super Optimal broth

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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grade

Molecular Biology

Quality Level

500

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO₄, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

Related Categories

Cloning & Expression

Industrial Microbiology

Microbial Culture Media

Molecular Cloning & Protein Expression

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:

Filter-sterilized, ready-to-use format
Convenient package sizes
Standard formulation

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动物来源培养基

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beta-Lactam resistance in salmonella strains isolated from retail meats in the United States by the National Antimicrobial Resistance Monitoring System between 2002 and 2006.

S Zhao et al.

Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)

Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)

Identification of Pathogenicity-Associated Loci in Klebsiella pneumoniae from Hospitalized Patients.

Rebekah M Martin et al.

mSystems, 3(3) (2018-07-03)

Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients

Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers

Iqbal A

PLoS ONE (2015)

Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids.

Daniel C Volke et al.

Bio-protocol, 11(4), e3917-e3917 (2021-03-19)

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted

Culture of Escherichia coli in SOC medium improves the cloning efficiency of toxic protein genes.

Qiao-Yang Sun et al.

Analytical biochemistry, 394(1), 144-146 (2009-07-23)

In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.

View All Related Papers

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

细菌转化实验方案

感受态细胞生长及其转化（吸收DNA）的通用实验方案。

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Global Trade Item Number

SKU	GTIN
S1797-10X5ML	04061838262424
S1797-5ML	04061838108456
S1797-100ML	04061838262417
S1797-10ML	04061838080356

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