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Merck
CN

S2519

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, ELISA (i)
Citations:
13
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biological source

rabbit

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

soy

technique(s)

dot blot: 1:500, indirect ELISA: 1:5,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

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General description

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.
Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.
Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues.

Immunogen

renatured soy protein extracted from soy acetone powder with hot urea.

Application

Anti-Soy Protein antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • supershift analysis
  • fluorescent imaging
  • immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Biochem/physiol Actions

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

nwg

Storage Class

12 - Non Combustible Liquids

Regulatory Information

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J S Freeth et al.
Endocrinology, 139(1), 20-28 (1998-01-08)
We have previously described two families (H and M) with GH binding protein-positive Laron Syndrome (LS), proposed to have one or more post GHR signaling defects. In the present study, we have examined whether the signal transducers and activators of
Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato
Fernandes FL and Fernandes MES
Pesquisa Agropecuaria Brasileira, 50(5), 399-406 (2015)
Activation of the signal transducers and activators of transcription signaling pathway by growth hormone (GH) in skin fibroblasts from normal and GH binding protein-positive Laron Syndrome children
Freeth JS, et al.
Endocrinology, 139(1), 20-28 (1998)
Marion Busch et al.
The Journal of investigative dermatology, 128(4), 890-896 (2007-12-01)
Currently, most medical gloves are produced with a low content of natural rubber latex (NRL) protein. However, they may be substituted by proteins of foreign origin to maintain specific properties of the material. The aim of this study was to
Soy protein
Montgomery KS
The Journal of perinatal education, 12(3), 42-42 (2003)

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