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Merck
CN

S2563

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

Synonym(s):

SCDase

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.32
MDL number:
Technical Service
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Quality Segment

foreign activity

exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free, protease and sphingomyelinase, essentially free

shipped in

dry ice

storage temp.

−20°C

General description

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.

Application

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.

Biochem/physiol Actions

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.
Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.

Physical form

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

Other Notes

One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C.


Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

nwg



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