S2628
N-Succinyl-L -phenylalanine-p-nitroanilide
protease substrate, ≥98% (TLC), powder
Synonym(s):
Suc-Phe-pNA
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About This Item
Empirical Formula (Hill Notation):
C19H19N3O6
CAS Number:
Molecular Weight:
385.37
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
219-472-6
MDL number:
Product Name
N-Succinyl-
Quality Segment
assay
≥98% (TLC)
form
powder
solubility
dioxane: 25 mg/mL, clear, colorless to faintly yellow
storage temp.
−20°C
SMILES string
OC(=O)CCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)N(=O)=O
InChI
1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1
InChI key
KNBLWBFJHZHYFG-INIZCTEOSA-N
General description
Reported to be a substrate for chymotrypsin.
Application
N-Succinyl-L -phenylalanine-p-nitroanilide has been used as a substrate for the hydrolytic activity of chymotrypsin and molar absorptive experiments. It has also been used for monitoring in vitro α-chymotrypsin inhibition assay.
Biochem/physiol Actions
N-Succinyl-L -phenylalanine-p-nitroanilide, a substrate for the hydrolytic activity of chymotrypsin, forms a yellow chromophore, p-nitroaniline, which can be measured spectrophotometrically at 410 nm.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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