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Merck
CN

S3504

Sigmacell Cellulose

Type 20, 20 μm

Synonym(s):

Cellulose, Cellulose powder, Cotton linters

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About This Item

CAS Number:
9004-34-6
UNSPSC Code:
12352200
NACRES:
NA.56
EC Number:
232-674-9
MDL number:
MFCD00081512

Key Documents

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Product Name

Sigmacell Cellulose, Type 20, 20 μm

SMILES string

O1[C@H](C(C(C(C1CO)O)O)O)O[C@@H]2C(OC(C(C2O)O)O)CO

InChI key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

type

Type 20

particle size

20 μm

Quality Level

200

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Application

High purity cellulose powders for partition chromatography.
Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.

Preparation Note

For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.

Legal Information

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000502867
0000502867
0000503788
0000503787
0000503788

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Stuart M Linton et al.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also
Nasrullah Shah et al.
Carbohydrate polymers, 98(2), 1585-1598 (2013-09-24)
Bacterial cellulose (BC) has received substantial interest owing to its unique structural features and impressive physico-mechanical properties. BC has a variety of applications in biomedical fields, including use as biomaterial for artificial skin, artificial blood vessels, vascular grafts, scaffolds for
Rachel A Burton et al.
Current opinion in biotechnology, 26, 79-84 (2014-04-01)
Plant cell walls consist largely of cellulose, non-cellulosic polysaccharides and lignin. Concerted attempts are underway to convert wall polysaccharides from crop plant residues into renewable transport fuels and other valuable products, and to exploit the dietary benefits of cereal grain
Okako Omadjela et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(44), 17856-17861 (2013-10-16)
Cellulose is a linear extracellular polysaccharide. It is synthesized by membrane-embedded glycosyltransferases that processively polymerize UDP-activated glucose. Polymer synthesis is coupled to membrane translocation through a channel formed by the cellulose synthase. Although eukaryotic cellulose synthases function in macromolecular complexes
Inka Vanwonterghem et al.
The ISME journal, 8(10), 2015-2028 (2014-04-18)
A replicate long-term experiment was conducted using anaerobic digestion (AD) as a model process to determine the relative role of niche and neutral theory on microbial community assembly, and to link community dynamics to system performance. AD is performed by
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Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

纤维素酶的酶学测定

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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