S3917
Single-strand Binding Protein from Escherichia coli
>95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity
Synonym(s):
SSB
Product Name
Single-strand Binding Protein from Escherichia coli, >95% (SDS-PAGE), Molecular Biology, Single strand binding protein to enhance PCR specificity
grade
Molecular Biology
assay
>95% (SDS-PAGE)
form
liquid
color
colorless
foreign activity
DNase and RNase, none detected
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
Biochem/physiol Actions
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.
General description
Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
Application
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk
WGK 2
Regulatory Information
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Structure of the SSB--DNA polymerase III interface and its role in DNA replication
Marceau AH, et al.
The Embo Journal, 30(20), 4236-4247 (2011)
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.
Q Chou
Nucleic acids research, 20(16), 4371-4371 (1992-08-25)
The Integron Integrase Efficiently Prevents the Melting Effect of Escherichia coli Single-Stranded DNA-Binding Protein on Folded attC Sites
Loot C, et al.
Journal of Bacteriology, 196(4), 762-771 (2014)
Binding Specificity of E. coli SSB protein for the ? subunit of DNA pol III Holoenzyme and PriA helicase
Alexander GK, et al.
Biochemistry, 49(17), 3555-3556 (2010)
J Kieleczawa et al.
Science (New York, N.Y.), 258(5089), 1787-1791 (1992-12-11)
When template DNA is saturated with a single-stranded DNA binding protein (SSB), strings of three or four contiguous hexanucleotides (hexamers) can cooperate through base-stacking interactions to prime DNA synthesis specifically from the 3' end of the string. Under the same
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