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S400HR

Sephacryl® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00165824
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Product Name

Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)

Quality Level

200

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

10-2000 kDa MW range (dextrans), 20-8000 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S400HR-750ML′s updated product is GE17-0609-01

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2518
MKCN9954
MKCJ0342
MKCG9384
MKCF7412

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Masaharu Tsuji et al.
International journal of systematic and evolutionary microbiology, 69(4), 944-948 (2019-01-04)
Four strains isolated from sediment sampled at the front of a retreating glacier on northern Ellesmere Island in the Canadian high Arctic, namely JCM 32575T, JCM 32576, JCM 32577 and JCM 32578, belong to a novel psychrophilic basidiomycetous yeast species
Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
Sinu P John et al.
Journal of virology, 81(17), 8967-8976 (2007-06-15)
The Ebola virus (EBOV) genome encodes for several proteins that are necessary and sufficient for replication and transcription of the viral RNAs in vitro; NP, VP30, VP35, and L. VP30 acts in trans with an RNA secondary structure upstream of
Hypolipidemic and antioxidant activity of enoki mushrooms (Flammulina velutipes)
Ming-Yei Y, et al.
BioMed Research International (2014)
An amphipathic a-helix is the principle membrane-embedded region of CTP:phosphocholine cytidylyltransferase. Identification of the 3-(trifluoromethyl)-3-(m-[<SUP>125</SUP>I]iodophenyl)diazirine photolabeled domain.
Johnson, J.E., et al.
Biochimica et Biophysica Acta - Biomembranes, 1324(2), 273-284 (1997)
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Global Trade Item Number

SKUGTIN
S400HR-250ML04061836924539
S400HR-750ML04061836973988
S400HR-100ML04061836973964

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