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S400HR

Sephacryl® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00165824

Key Documents

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Product Name

Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

Quality Level

200

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Application

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.

General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S400HR-750ML′s updated product is GE17-0609-01

Legal Information

Sephacryl is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2518
MKCN9954
MKCJ0342
MKCG9384
MKCF7412

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H A Davies et al.
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Rapid Detection of Adenovirus from Fecal Specimens.
Tabish, T., et al. et al.
Methods in Molecular Medicine, 12, 1-6 (1998)
Hypolipidemic and antioxidant activity of enoki mushrooms (Flammulina velutipes)
Ming-Yei Y, et al.
BioMed Research International (2014)
Bertrand Vileno et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(20), 8218-8223 (2011-05-04)
Double electron electron resonance EPR methods was used to measure the effects of the allosteric modulators, phosphorylation, and ATP, on the distances and distance distributions between the two regulatory light chain of myosin (RLC). Three different states of smooth muscle
An amphipathic a-helix is the principle membrane-embedded region of CTP:phosphocholine cytidylyltransferase. Identification of the 3-(trifluoromethyl)-3-(m-[<SUP>125</SUP>I]iodophenyl)diazirine photolabeled domain.
Johnson, J.E., et al.
Biochimica et Biophysica Acta - Biomembranes, 1324(2), 273-284 (1997)
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