S500HR
Sephacryl® Size Exclusion Resin
500-HR, MW range 40-20,000 kDa (dextrans)
Synonym(s):
Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
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About This Item
Product Name
Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)
Quality Level
description
stability: stable to 0.2M NaOH
form
suspension (20% aqueous ethanol)
feature
autoclavable
matrix
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
particle size
25-75 μm (wet)
pore size
40-20,000 kDa MW range (dextrans)
compatibility
mode of use size exclusion chromatography
storage temp.
2-8°C
General description
- Supplied swollen, suspended in 20% aqueous ethanol
- Autoclavable
- Stable to 0.2N sodium hydroxide (NaOH)
- Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01
Application
Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
Legal Information
Sephacryl is a registered trademark of Cytiva
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
100.4 - 109.4 °F - closed cup
flash_point_c
38 - 43 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Regulatory Information
危险化学品
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A Lamberg et al.
The Journal of biological chemistry, 271(20), 11988-11995 (1996-05-17)
An efficient expression system for recombinant collagens would have numerous scientific and practical applications. Nevertheless, most recombinant systems are not suitable for this purpose, as they do not have sufficient amounts of prolyl 4-hydroxylase activity. Pro-alpha 1 chains of human
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.
New structural features of Acacia tortuosa gum exudate
Martinez M, et al.
Food Chemistry, 182, 105-110 (2015)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S500HR-100ML | 04061836974039 |
| S500HR-250ML | 04061836935054 |