S5394
Lipoprotein Deficient Serum from fetal calf
frozen liquid
Synonym(s):
Fetal Calf Serum, Lipoprotein Deficient Serum
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About This Item
NACRES:
NA.61
UNSPSC Code:
12352207
Biological source:
bovine fetus
Form:
frozen liquid
Shipped in:
dry ice
biological source
bovine fetus
Quality Level
form
frozen liquid
concentration
≥14 mg protein/mL Biuret
impurities
<1 ng/mg protein endotoxin, <5 % of normal lipoprotein
shipped in
dry ice
storage temp.
−20°C
General description
Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.
Application
Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).
Preparation Note
0.2 μm filtered
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
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Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Maria Inasu et al.
NPJ breast cancer, 7(1), 127-127 (2021-09-25)
27-hydroxycholesterol (27HC), synthesized from cholesterol by the enzyme CYP27A1, differentially impacts estrogen receptor positive (ER+) breast cancer (BC) cell growth depending on estrogen levels. This study examined the association between CYP27A1 expression and prognosis in a cohort of 193 premenopausal
Protocols
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S5394-50ML | 04061838256379 |
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