S6147
Sephadex® G-100
DNA grade, BioReagent, Molecular Biology, medium
Product Name
Sephadex® G-100, DNA grade, BioReagent, Molecular Biology, medium
grade
Molecular Biology
product line
BioReagent
form
beads
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 9-11 mL
bead diameter
40-120 μm
pore size
exclusion limit (25 bp dsDNA; (rA)27)
pH
2-10
application(s)
life science and biopharma
compatibility
Cytiva
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
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Application
Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
General description
Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Legal Information
Sephadex is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
S Kaushik et al.
Methods in molecular biology (Clifton, N.J.), 445, 227-244 (2008-04-22)
Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian cells able to selectively degrade cytosolic proteins in lysosomes. CMA is maximally activated in response to stressors such as prolonged starvation, exposure to toxic compounds, or oxidative stress. We
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Dae-Young Lee et al.
Journal of medicinal food, 15(4), 399-405 (2012-02-10)
Grains of sugary rice were extracted with 80% aqueous methanol, and the concentrated extracts were successively partitioned using ethyl acetate, n-butanol, and water. From the n-butanol fractions, four flavonoid glycosides were isolated through repeated silica gel, octadecyl silica gel, and
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