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S6510

N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin

Name: N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin
Brand: SIGMA

≥90% (HPLC), Chymotrypsin substrate, powder

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About This Item

Empirical Formula (Hill Notation):

C₄₀H₅₃N₅O₁₀

CAS Number:

94367-21-2

Molecular Weight:

763.88

UNSPSC Code:

12352200

PubChem Substance ID:

24899709

NACRES:

NA.77

MDL number:

MFCD00080248

Assay:

≥90% (HPLC)

Form:

powder

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Properties

Product Name

N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin, ≥90% (HPLC)

Quality Segment

200

assay

≥90% (HPLC)

form

powder

solubility

0.1% trifluoroacetic acid in acetonitrile: water (3:1): 1 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

CC(C)CC(NC(=O)CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(Cc1ccc(O)cc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C40H53N5O10/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49)

InChI key

UVFAEQZFLBGVRM-UHFFFAOYSA-N

Description

General description

Fluorogenic substrate for chymotrypsin-like enzymes, such as cathepsin B and calpain which have been implicated in programmed cell death.

Application

N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin was used in proteasome chymotrypsin-like activity assay in Jurkat cell lysate⁶ and crude cell lysate of rice.⁷

Biochem/physiol Actions

In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.⁶

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

hcodes

H413

pcodes

P273

Hazard Classifications

Aquatic Chronic 4

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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