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Merck
CN

S7025

50 bp DNA Step Ladder

for DNA electrophoresis

Synonym(s):

DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Key Documents

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Quality Level

100

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Other Notes

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
SLCP7866
SLCM8340
SLCF7407
SLCD4724
SLCD0018

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Alper Dede et al.
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Soil actinomycetes are a highly common group of bacteria and frequently studied as having secondary metabolites in the potential of producing the most preferred antagonistic content. Considering the continuous variation in soil structure, there is a potential for encountering different
Rhodotorula portillonensis sp. nov., a basidiomycetous yeast isolated from Antarctic shallow-water marine sediment.
Laich F, et al.
International Journal of Systematic and Evolutionary Microbiology, 63, 3884-3891 (2013)

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

Related Content

Data Sheet - S7025

Instructions

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