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Merck
CN

S7388

Sigma-Aldrich

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide

Subtilisin substrate

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide, N-Succinyl-Ala-Ala-Pro-Phe-pNA

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About This Item

Empirical Formula (Hill Notation):
C30H36N6O9
CAS Number:
70967-97-4
Molecular Weight:
624.64
Beilstein:
4289896
MDL number:
MFCD00077161
UNSPSC Code:
12352200
PubChem Substance ID:
24899742
NACRES:
NA.32

Key Documents

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Product Name

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide,

Quality Level

300

storage temp.

−20°C

SMILES string

[H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(O)=O)C(=O)N[C@@H](Cc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C30H36N6O9/c1-18(31-25(37)14-15-26(38)39)27(40)32-19(2)30(43)35-16-6-9-24(35)29(42)34-23(17-20-7-4-3-5-8-20)28(41)33-21-10-12-22(13-11-21)36(44)45/h3-5,7-8,10-13,18-19,23-24H,6,9,14-17H2,1-2H3,(H,31,37)(H,32,40)(H,33,41)(H,34,42)(H,38,39)/t18-,19-,23-,24-/m0/s1

InChI key

LKDMKWNDBAVNQZ-WJNSRDFLSA-N

Gene Information

human ... FKBP10(60681)

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Amino Acid Sequence

N-Suc-Ala-Ala-Pro-Phe-pNA

General description

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a colorimetric substrate for chymotrypsin, human leukocyte cathepsin G and peptidyl prolyl isomerase.

Application

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.
N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used:
  • as a substrate for cathepsin G assay in bone marrow lysates.
  • in proteolytic activity assay of serine protease subtilisin carlsberg (SC).
  • as a substrate for neutrophil elastase, trypsin and chymotrypsin in protease assay.

Biochem/physiol Actions

N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a substrate for subtilisin and proteases.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000438426
0000438426
0000438438
0000438438
0000420705

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Improved Enantioselectivity of Subtilisin Carlsberg towards Secondary Alcohols by Protein Engineering
Dorau R, et al.
Chembiochem, 19(4), 338-346 (2018)
J J Siekierka et al.
Nature, 341(6244), 755-757 (1989-10-26)
CYCLOSPORIN A and the newly discovered immunosuppressant, FK-506, are potent inhibitors of T cell activation. In addition to their clinical importance in the prevention of allograft rejection, cyclosporin A and FK-506 represent important reagents for the study of the molecular
Interleukin-33 is activated by allergen-and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage
Scott IC, et al.
Scientific Reports, 8(1), 3363-3363 (2018)
Jolanda Neef et al.
Microbial cell factories, 16(1), 57-57 (2017-04-06)
The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major
Marco Bonelli et al.
International journal of molecular sciences, 21(14) (2020-07-17)
Modulation of nutrient digestion and absorption is one of the post-ingestion mechanisms that guarantees the best exploitation of food resources, even when they are nutritionally poor or unbalanced, and plays a pivotal role in generalist feeders, which experience an extreme
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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

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