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Merck
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S7571

Superoxide Dismutase from bovine erythrocytes

greener alternative

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

Synonym(s):

CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase

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About This Item

CAS Number:
9054-89-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-943-0
MDL number:
MFCD00132404
EC Number:
1.15.1.1 (BRENDA, IUBMB)

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Product Name

Superoxide Dismutase from bovine erythrocytes, lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

biological source

bovine

form

lyophilized powder

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

composition

Protein, ≥95% biuret

storage condition

(Store under nitrogen.
Tightly closed. Dry.)

greener alternative product characteristics

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
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sustainability

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technique(s)

immunoblotting: suitable
inhibition assay: suitable

color

blue-green

pI 

4.95

solubility

water: 20 mg/mL
aqueous buffer, pH 7.5: soluble

UniProt accession no.

P00442
P41976

application(s)

diagnostic assay manufacturing

greener alternative category

, Re-engineered

storage temp.

−20°C

Quality Level

300

Gene Information

cow ... SOD1(281495), SOD2(281496)

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Other Notes

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Physical form

Lyophilized powder, essentially salt-free

Application

Superoxide dismutase from bovine erythrocytes has been used:

  • in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
  • in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
  • to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
  • in combination with catalase to study its effect on cell differentiation in vitro
  • to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

Biochem/physiol Actions

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

General description

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000520165
0000520191
0000520191
0000520165
0000494458

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Superoxide-Mediated Upregulation of MMP9 Participates in BMPR2 Destabilization and Pulmonary Hypertension Development
Antioxidants, 12(11), 1961-1961 (2023)
Tomohiro Furukawa et al.
Toxins, 11(2) (2019-02-15)
Aflatoxin contamination of crops is a worldwide problem, and elucidation of the regulatory mechanism of aflatoxin production, for example relative to the oxidative⁻antioxidative system, is needed. Studies have shown that oxidative stress induced by reactive oxygen species promotes aflatoxin production.
Superoxide dismutase and catalase significantly improve the osteogenic differentiation potential of osteogenetically compromised human adipose tissue-derived stromal cells in vitro
Sahlender B, et al.
Stem Cell Research (2022)
Michael A Hough et al.
Structure (London, England : 1993), 11(8), 937-946 (2003-08-09)
Copper zinc superoxide dismutase (CuZnSOD) forms a crucial component of the cellular response to oxidative stress by catalyzing the dismutation of the superoxide radical to hydrogen peroxide and water. Mutations in human CuZnSOD are associated with the development of familial
Possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction.
F Hirata et al.
The Journal of biological chemistry, 246(24), 7825-7826 (1971-12-25)
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Articles

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

线粒体应激和ROS

氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。

Protocols

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

Separation of Superoxide dismutase

超氧化物歧化酶的酶学测定

在测定超氧化物歧化酶活性时，使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50％。

Related Content

Data Sheet - S7571

Instructions

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