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S7632

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide

Name: N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide
Brand: SIGMA

protease substrate

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₃₄N₆O₉

CAS Number:

108322-03-8

Molecular Weight:

550.56

NACRES:

NA.32

PubChem Substance ID:

24899748

UNSPSC Code:

12352204

MDL number:

MFCD00133618

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Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

DMF: 10 mg/mL, clear, colorless

UniProt accession no.

P57774

storage temp.

−20°C

SMILES string

CC(C)C(NC(=O)C(C)NC(=O)C(C)NC(=O)CCC(O)=O)C(=O)NC(C)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C24H34N6O9/c1-12(2)20(24(37)27-14(4)22(35)28-16-6-8-17(9-7-16)30(38)39)29-23(36)15(5)26-21(34)13(3)25-18(31)10-11-19(32)33/h6-9,12-15,20H,10-11H2,1-5H3,(H,25,31)(H,26,34)(H,27,37)(H,28,35)(H,29,36)(H,32,33)

InChI key

IYHHIHIBYVIDMJ-UHFFFAOYSA-N

Gene Information

mouse ... Npy(109648)

General description

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide is a chromogenic and a synthetic peptide substrate for proteases, elastase and subtilisin.

Application

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide has been used as a synthetic peptide substrate for:

Bacillus pumilus alkaline protease (DHAP)
mesophilic subtilisin Carlsberg (sC) to assess the hydrolytic activity of wild-type and mutant sC
Pseudoalteromonas arctica cold-active protease (Pro21717)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Substrate specificity and thermostability of the dehairing alkaline protease from Bacillus pumilus.

Min-Yuan Wan et al.

Applied biochemistry and biotechnology, 159(2), 394-403 (2009-01-10)

An alkaline protease (DHAP) from Bacillus pumilus has shown great potential in hide dehairing. To get better insights on its catalytic properties for application, the substrate specificity and thermostability were investigated using five natural proteins and nine synthetic peptides. The

A Zingerone Analog, Acetyl Zingerone, Bolsters Matrisome Synthesis, Inhibits Matrix Metallopeptidases, and Represses IL-17A Target Gene Expression.

William R Swindell et al.

The Journal of investigative dermatology, 140(3), 602-614 (2019-08-30)

Zingerone (Z) is a phenolic alkanone derived from natural sources with anti-inflammatory and antioxidant effects. Acetyl zingerone (AZ) is a recently designed molecule that shares structural features with Z but is expected to have improved stability and antioxidant function. This

Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4 Å resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme.

Ha Ju Park et al.

PloS one, 13(2), e0191740-e0191740 (2018-02-22)

Enzymes isolated from organisms found in cold habitats generally exhibit higher catalytic activity at low temperatures than their mesophilic homologs and are therefore known as cold-active enzymes. Cold-active proteases are very useful in a variety of biotechnological applications, particularly as

Gly or Ala substitutions for Pro(210)Thr(211)Asn(212) at the β8-β9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability.

Naoki Fuchita et al.

Biochimica et biophysica acta, 1824(4), 620-626 (2012-02-14)

A comparison of the primary structures among psychrophilic, mesophilic, and thermophilic subtilases revealed that the turn between the β8 and β9 strands (β8-β9 turn, BPN' numbering) of psychrophilic subtilases are more flexible than those of their mesophilic and thermophilic counterparts.

Subtilisin enzymes: Practical protein engineering, 379 (2013)

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Global Trade Item Number

SKU	GTIN
S7632-25MG	04061832925455

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