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S7897

Sarcosine Oxidase from Bacillus sp.

lyophilized powder, 25-50 units/mg solid

Synonym(s):

Sarcosine: oxygen oxidoreductase (demethylating)

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About This Item

CAS Number:
9029-22-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-850-5
MDL number:
MFCD00132221
EC Number:
1.5.3.1 (BRENDA, IUBMB)
Specific activity:
25-50 units/mg solid
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form

lyophilized powder

Quality Segment

specific activity

25-50 units/mg solid

mol wt

44 kDa

storage temp.

−20°C

General description

Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.
Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline.

Application

Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.
Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins.

Physical form

No stabilizers added

Other Notes

One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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