S8820
SIGMAFAST™ Protease Inhibitor Tablets
For General Use
biological source
bovine lung
bovine milk
Quality Level
form
tablet
solubility
water: 1 tablet/100mL, clear to hazy, colorless
storage temp.
2-8°C
Related Categories
General description
Specificity
Application
- to wash raw semen for membrane protein analysis
- in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
- as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
- as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)
Features and Benefits
- Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
- Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
- Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
- Tested and optimized for general use: proven performance and reliability for a wide range of applications.
Components
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Quantity
Physical form
Other Notes
Legal Information
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Example:
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705578-5MG-PW
PL860-CGA/SHF-1EA
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1000309185
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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.
Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).
Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with ELISA?
Yes, because it is water based.
How should I dissolve Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.
What is the concentration of the inhibitors in Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?
When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF 2 mME-64 14 μMBestatin 130 μMLeupeptin 0.9 μMAprotinin 0.3 μMEDTA 1 mM
How much of Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, needs to be used in cell lysates?
One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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