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S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
477-73-6
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899820
EC Number:
207-518-8
MDL number:
MFCD00011759
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
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Product Name

Safranin O, certified by the BSC

agency

certified by the BSC

Quality Segment

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Application

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.
Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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