Anti-HIF1A antibody produced in rabbit

Recombinant fragment contain a sequence corresponding to a region within amino acids 657 and 826 of HIF1 alpha

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-Fr: Assay-dependent dilution, IHC-P: 1:100-1:1000, IP: 1:100-1:570, WB: 1:500-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

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1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

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