SAB4200119
Monoclonal Anti-FLAG-Peroxidase antibody produced in rat
2-4 mg/mL, clone 6F7, purified immunoglobulin
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
6F7, monoclonal
Application:
FLAG purification, WB
Citations:
9
biological source
rat
Quality Segment
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F7, monoclonal
form
buffered aqueous solution
species reactivity
all
concentration
2-4 mg/mL
technique(s)
FLAG purification: suitable, western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.
The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.
Immunogen
purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).
Application
Learn more product details in our FLAG® applications portal.
Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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