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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
Clone 1A6C2, monoclonal
Application:
ICC, IHC, WB
Citations:
7
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Clone 1A6C2, monoclonal
form
buffered aqueous solution
mol wt
~72 kDa
species reactivity
human, rat, monkey, mouse
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: 2.5-5.0 μg/mL using L929, HeLa, 3T3 and COS-7 cell extracts
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SEPT9(10801)
mouse ... Sept9(53860)
rat ... Sept9(83788)
General description
Monoclonal Anti-Septin 9 (rat IgG2a isotype) is derived from the hybridoma 1A6C2 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a synthetic peptide of corresponding to the N-terminus of rat Septin 9, conjugated to KLH. Septin 9 (SEPT9) is a member of the septin family (also designated MSF/eseptin/Sint1). This gene is mapped to human chromosome 17q25.
Immunogen
synthetic peptide of corresponding to the N-terminus of rat Septin 9 conjugated to KLH
Application
Monoclonal Anti-Septin 9 has been used in:
- immunoblotting
- immunocytochemistry
- immunohistochemistry
- immunofluorescence
Biochem/physiol Actions
Septin 9 (SEPT9) has been implicated in tumorigenesis. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. In addition, SEPT9 is shown to be a DNA methylation-based biomarker, effectively discriminated colorectal cancer from normal specimens.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
常规特殊物品
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Related Content
Instructions
Taro Tachibana et al.
Hybridoma (2005), 29(2), 169-171 (2010-05-07)
The septin family of GTPase proteins has been shown to be important for cell division, cytoskeletal organization, and membrane-remodeling events. Septin 9 (SEPT9) is a member of the septin family (also designated MSF/eseptin/Sint1) and has been implicated in tumorigenesis. The
Polarity of neuronal membrane traffic requires sorting of kinesin motor cargo during entry into dendrites by a microtubule-associated septin
Karasmanis EP, et al.
Developmental Cell, 46(2), 204-218 (2018)
Eva P Karasmanis et al.
Current biology : CB, 29(13), 2174-2182 (2019-06-18)
Abscission is the terminal step of mitosis that physically separates two daughter cells [1, 2]. Abscission requires the endocytic sorting complex required for transport (ESCRT), a molecular machinery of multiple subcomplexes (ESCRT-I/II/III) that promotes membrane remodeling and scission [3-5]. Recruitment
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4200191-200UL | 04061837868603 |