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SAB4200341

Anti-Golph3 (N-terminal) antibody produced in rabbit

Name: Anti-Golph3 (N-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-GOPP1, Anti-GPP34, Anti-Golgi peripheral membrane protein 1, 34 kDa, Anti-Golgi phosphoprotein 3 (coat-protein), Anti-Golgi-associated protein; Mitochondrial DNA absence factor, Anti-MIDAS

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~34 kDa

species reactivity

mouse, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells, indirect immunofluorescence: 2.5-5.0 μg/mL using mouse 3T3 cells, western blot: 1-2 μg/mL using whole extracts of human HeLa cells

UniProt accession no.

Q9H4A6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

Description

General description

Golph3 (Golgi phosphoprotein 3), also known as coat protein GPP34, is a peripheral membrane protein of the Golgi stack, that localizes to the trans-Golgi network. It is an oncogene that is commonly targeted for amplification in human cancer and in cancer cell lines.

Immunogen

peptide corresponding to the N-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and bovine.

Application

Anti-Golph3 (N-terminal) antibody produced in rabbit has been used in:

immunoprecipitation
immunofluorescence
immunoblotting

Biochem/physiol Actions

Golph3 (Golgi phosphoprotein 3) is important for Golgi trafficking and morphology by interacting with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. It is a phosphatidylinositol-4-phosphate (PtdIns(4)P binding protein required for Golgi function. GOLPH3 overexpression is correlated with hyperactivation of mTOR signaling, in human cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

低风险生物材料

This item has

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Certificates of Analysis (COA)

Lot/Batch Number

0000161511

0000150774