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Merck
CN

SAB4200415

Anti-NEZHA antibody, Mouse monoclonal

clone NEZHA-1, purified from hybridoma cell culture

Synonym(s):

Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA, Monoclonal Anti-NEZHA antibody produced in mouse

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-NEZHA antibody, Mouse monoclonal, clone NEZHA-1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NEZHA-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

canine, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells

isotype

IgG1

UniProt accession no.

Q9P1Y5

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CAMSAP3(57662)
mouse ... Camsap3(69697)

Application

Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.
Monoclonal Anti-NEZHA antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • immunofluorescence
  • mass-spectrometry-based analysis of a streptavidin pull-down assay
  • immunostaining

Biochem/physiol Actions

CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.
NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.
NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000154703
0000154703
0000145861
0000145861
012M4797

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CAMSAP3-dependent microtubule dynamics regulates Golgi assembly in epithelial cells.
Wang J
Journal of Genetics and Genomics = Yi Chuan Xue Bao, 44, 39-49 (2017)
Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling
Kourtidis A, et al.
The Journal of cell biology, 216(10), 3073-3085 (2017)
CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells.
Toya M
Proceedings of the National Academy of Sciences of the USA, 113, 332-337 (2016)
Laure Coquand et al.
The Journal of cell biology, 220(8) (2021-05-22)
Neurons of the neocortex are generated by stem cells called radial glial cells. These polarized cells extend a short apical process toward the ventricular surface and a long basal fiber that acts as a scaffold for neuronal migration. How the
CAMSAP3 maintains neuronal polarity through regulation of microtubule stability
Pongrakhananon V, et al.
Proceedings of the National Academy of Sciences of the USA, 115(39), 9750-9755 (2018)
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