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Merck
CN

SAB4200689

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

Synonym(s):

Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal, clone 5C5, hybridoma cell culture supernatant

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5C5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

packaging

antibody small pack of 25 μL

technique(s)

ELISA: suitable
immunoblotting: 1:500-1:1000 using rat heart tissue extracts
immunofluorescence: 1:500-1000 using human HeLa cells.
immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA2(59)

Related Categories

Application

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
  • indirect immunofluorescence staining
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunofluorescence

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
069M4811V
0000131337
0000127271
018M4835V
22190421

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PEDF decreases cardiomyocyte edema during oxygen-glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1
Huang B, et al.
International Journal of Molecular Medicine, 43(5), 1979-1990 (2019)
The actin cytoskeleton
Molecular and Cellular Biology, 1979-1990 (2000)
Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles
Miklavc P, et al.
Journal of Cell Science, 128(6), 1193-1203 (2015)
Na Tang et al.
Nature communications, 13(1), 7455-7455 (2022-12-03)
Intracellular Ca2+ dysregulation is a key marker in septic cardiac dysfunction; however, regulation of the classic Ca2+ regulatory modules cannot successfully abolish this symptom. Here we show that the knockout of transient receptor potential canonical (TRPC) channel isoforms TRPC1 and
Xinhui Fan et al.
Frontiers in pharmacology, 13, 892643-892643 (2022-07-23)
Diabetes mellitus (DM) often involves cardiovascular complications; however, treatment regimens are limited. ROCK1 (rho-associated coiled-coil containing protein kinase 1) serves as a pathological factor in several diabetic complications. Herein, we aimed to explore the effect of Fasudil (a ROCK1 inhibitor)
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