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SAB4200725

Anti-Splicing Factor SC-35 antibody, Mouse monoclonal

Name: Anti-Splicing Factor SC-35 antibody, Mouse monoclonal
Brand: SIGMA

clone SC-35, purified from hybridoma cell culture

Synonym(s):

Anti-PR264, Anti-SFRS2, Anti-SFRS2A, Anti-SRp30b, Anti-Splicing component 35 kDa (SC35), Anti-serine/arginine-rich splicing factor 2 (SRSF2)

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

SC-35, monoclonal

Application:

DB, ELISA, IF, IHC, IP

Citations:

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SC-35, monoclonal

form

buffered aqueous solution

mol wt

~35 kDa

species reactivity

human, mouse, quail, hamster, frog, porcine, rat

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable, dot blot: suitable, immunoblotting: suitable, immunofluorescence: 0.125-0.25 μg/mL using HeLa cells, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

UniProt accession no.

Q01130

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SRSF2(6427)

General description

Anti-Splicing Factor SC-35 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a RBF-DHJ mouse immunized with partially purified spliceosomes prepared from HeLa cells. Splicing Factor SC-35, also known as serine/arginine rich splicing factor 2 (SRSF2) belongs to the family of of serine/arginine-rich (SR) proteins.

Immunogen

partially purified spliceosomes prepared from HeLa cells

Application

Anti-Splicing Factor SC-35 antibody has been used in

dot blot
enzyme linked immunosorbent assay (ELISA)
immunofluorescence
immunohistochemistry
immunoblotting
immunoprecipitation

Biochem/physiol Actions

Splicing Factor SC-35 are critical regulators of constitutive and alternative pre-mRNA splicing. It also controls cellular apoptosis in response to genotoxic stress. Serine/arginine protein kinase 1 (SRPK1) specifically phosphorylates SC-35, resulting in p53 suppression and cyclin D1 upregulation. Elevated levels of SC-35 correlate with aggressive phenotype in several cancer types including lung squamous cell carcinoma and lung adenocarcinoma. SC-35 has oncogenic function in tumor progression of human papilloma virus (HPV)-infected cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000444885

0000415592

0000388894

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E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35

Merdzhanova L, et al.

Cell Death and Differentiation, 15(12), 1815-1815 (2008)

Knockdown of Son, a mouse homologue of the ZTTK syndrome gene, causes neuronal migration defects and dendritic spine abnormalities.

Masashi Ueda et al.

Molecular brain, 13(1), 80-80 (2020-05-26)

Zhu-Tokita-Takenouchi-Kim (ZTTK) syndrome, a rare congenital anomaly syndrome characterized by intellectual disability, brain malformation, facial dysmorphism, musculoskeletal abnormalities, and some visceral malformations is caused by de novo heterozygous mutations of the SON gene. The nuclear protein SON is involved in

Revealing RCOR2 as a regulatory component of nuclear speckles.

Carlos Rivera et al.

Epigenetics & chromatin, 14(1), 51-51 (2021-11-26)

Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance

Nuclear speckle integrity and function require TAO2 kinase.

Shengyan Gao et al.

Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2206046119-e2206046119 (2022-06-16)

Nuclear speckles are non-membrane-bound organelles known as storage sites for messenger RNA (mRNA) processing and splicing factors. More recently, nuclear speckles have also been implicated in splicing and export of a subset of mRNAs, including the influenza virus M mRNA

Global Trade Item Number

SKU	GTIN
SAB4200725-100UL	04061838037794

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