SAB4200741
Anti-Human IgE antibody, Mouse monoclonal
clone GE-1, purified from hybridoma cell culture
Synonym(s):
Anti-Immunoglubulin E
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
GE-1, monoclonal
Species reactivity:
human
Application:
ELISA, IP
Citations:
6
biological source
mouse
Quality Segment
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GE-1, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating, immunoblotting: suitable, immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.
Immunogen
purified human IgE myeloma protein
Application
Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.
Biochem/physiol Actions
Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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