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SAB4200744

Anti-BDNF antibody, Mouse Monoclonal

Name: Anti-BDNF antibody, Mouse Monoclonal
Brand: SIGMA

clone BDNF129-13, purified from hybridoma cell culture

Synonym(s):

Anti-Abrineurin, Anti-Brain-derived neurotrophic factor

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

BDNF129-13, monoclonal

Application:

ICC, IHC, IP

Citations:

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BDNF129-13, monoclonal

form

buffered aqueous solution

mol wt

~12 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein, immunocytochemistry: suitable, immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections, immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein

isotype

IgG2b

UniProt accession no.

P23560

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BDNF(627)

Description

General description

Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).

Immunogen

Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH

Application

Anti-BDNF antibody, Mouse Monoclonal has been used in:

immunoblotting
immunohistochemistry
immunoprecipitation
immunocytochemistry

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000206612

0000192649