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SAB4200746

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

Name: Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal
Brand: SIGMA

clone C3, purified from hybridoma cell culture

Synonym(s):

Anti-AFP, Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin

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About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

C3, monoclonal

Application:

DB, ELISA, IF, IHC

Citations:

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Properties

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

C3, monoclonal

form

buffered aqueous solution

mol wt

~74 kDa

species reactivity

human, canine, porcine

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable, dot blot: suitable, immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract, immunofluorescence: suitable, immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections

isotype

IgG2a

UniProt accession no.

P02771

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AFP(174)

Description

General description

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

Immunogen

Purified human α-fetoprotein

Biochem/physiol Actions

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

037M4760V

51170105

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Global Trade Item Number

SKU	GTIN
SAB4200746-100UL	04061833350980