biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
C3, monoclonal
form
buffered aqueous solution
mol wt
~74 kDa
species reactivity
human, canine, porcine
concentration
~1.0 mg/mL
technique(s)
ELISA: suitable, dot blot: suitable, immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract, immunofluorescence: suitable, immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AFP(174)
General description
编码α-肽蛋白(AFP)的基因定位于人4号染色体。它编码一种分子量为70kDa的血浆糖蛋白。在胚胎卵黄囊和肝脏发育过程中,它是与血清白蛋白一起合成的。
Immunogen
纯化的人α-肽蛋白
Biochem/physiol Actions
α-肽蛋白(AFP)可参与到胎儿和增生的成人肝细胞中脂肪酸的调节。在急性肝损伤中发现其表达有所提高,表明活跃的肝再生。AFP与脂肪肝疾病(FLD)相关,而这是一种可能导致肝硬化和肝细胞癌的疾病。它可作为HCC(肝细胞癌)的一种肿瘤标志物。
Physical form
溶于含有 15 mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水(pH 7.4)溶液中。
Preparation Note
储存在–20°C。若需连续使用,可在2-8°C下储存最长一个月。若需延长储存时间,可将溶液分装并冷冻。不建议反复冻融。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完,则应丢弃。
Other Notes
为了在不同的技术和制备中获得最佳结果,我们建议通过效价测试确定最佳的工作浓度。
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Evaluation of alpha-fetoprotein in detecting hepatocellular carcinoma recurrence after radiofrequency ablation.
Siripongsakun S, et al.
Journal of Gastroenterology and Hepatology (2014)
Linkage of the evolutionarily-related serum albumin and alpha-fetoprotein genes within q11-22 of human chromosome 4.
M E Harper, et al.
American Journal of Human Genetics (1983)
Ana Gil-Bona et al.
International journal of molecular sciences, 24(21) (2023-11-14)
Excess albumin in enamel is a characteristic of the prevalent developmental dental defect known as chalky teeth or molar hypomineralization (MH). This study uses proteomic analyses of pig teeth to discern between developmental origin and post-eruptive contamination and to assess
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SAB4200746-100UL | 04061833350980 |