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SAB4200750

Anti-Gelsolin antibody, Mouse Monoclonal

Name: Anti-Gelsolin antibody, Mouse Monoclonal
Brand: SIGMA

clone GS-2C4, purified from hybridoma cell culture

Synonym(s):

Anit-AGEL, Anit-Actin-depolymerizing factor (ADF), Anit-GSN

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

GS-2C4, monoclonal

Application:

IF, IHC, IP

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GS-2C4, monoclonal

form

buffered aqueous solution

mol wt

~85 kDa

species reactivity

rabbit, canine, human, mouse, monkey

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1-2.5 μg/mL using MDCK cell line extract, immunofluorescence: suitable, immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections, immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P06396

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GSN(2934)

Description

General description

Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma).

Immunogen

Human plasma gelsolin

Application

Anti-Gelsolin antibody, Mouse Monoclonal has been used in:

immunoblotting
immunofluorescence
immunohistochemistry
immunoprecipitation

Biochem/physiol Actions

Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c