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SAB4200753

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal

clone AK-11, purified from hybridoma cell culture

Synonym(s):

Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1

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About This Item

NACRES:
NA.44
UNSPSC Code:
12352203
Clone:
AK-11, monoclonal
Species reactivity:
mouse, chicken, rat, human
Application:
IF, IP
Citations:
4

Key Documents

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biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AK-11, monoclonal

form

buffered aqueous solution

mol wt

~56 kDa

species reactivity

mouse, chicken, rat, human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract, immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells, immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line

isotype

IgG2a

UniProt accession no.

P31749

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr450)

Gene Information

human ... AKT1(207)

General description

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.

Immunogen

Synthetic peptide corresponding to human AKT1 pThr450

Application

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • immunoprecipitation

Biochem/physiol Actions

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).
Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
037M4764V

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Novel somatic and germline mutations in intracranial germ cell tumours
Wang L, et al.
Nature, 511(7508), 241-245 (2014)
Akt/PKB: one kinase, many modifications
Risso G, et al.
The Biochemical Journal, 468(2), 203-214 (2015)
The Akt pathway in oncology therapy and beyond
Nitulescu G M, et al.
International Journal of Oncology, 53(6), 2319-2331 (2018)
Signalling specificity in the Akt pathway in breast cancer
Clark A R and Toker A
Biochemical Society Transactions, 42(5), 1349-1355 (2014)

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