SAB4200753
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal
clone AK-11, purified from hybridoma cell culture
别名:
Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Clone:
AK-11, monoclonal
Species reactivity:
mouse, chicken, rat, human
Application:
IF, IP
Citations:
4
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
AK-11, monoclonal
form
buffered aqueous solution
mol wt
~56 kDa
species reactivity
mouse, chicken, rat, human
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract, immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells, immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pThr450)
Gene Information
human ... AKT1(207)
General description
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.
Immunogen
Synthetic peptide corresponding to human AKT1 pThr450
Application
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunoprecipitation
Biochem/physiol Actions
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).
Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Novel somatic and germline mutations in intracranial germ cell tumours
Wang L, et al.
Nature, 511(7508), 241-245 (2014)
Akt/PKB: one kinase, many modifications
Risso G, et al.
The Biochemical Journal, 468(2), 203-214 (2015)
The Akt pathway in oncology therapy and beyond
Nitulescu G M, et al.
International Journal of Oncology, 53(6), 2319-2331 (2018)
Signalling specificity in the Akt pathway in breast cancer
Clark A R and Toker A
Biochemical Society Transactions, 42(5), 1349-1355 (2014)
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